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During my second year at the Technical University of Budapest I met Prof Genő Fekete for the first time and was fascinated by his skills, knowledge and experiences. Before this encounter, I spent most of the semester on basketball courts and athletics tracks.

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Here, you’ll find all the things related to your computer’s system, including your display settings. It’s like the control center for your device, housing everything from sound options to notifications.

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We saw that friction related effects were more important for small molecule separations whilst pressure effects were much more significant for protein separations. Insulin was an attractive example as we could clearly see the decrease of retention in constant inlet pressure mode and the increase of retention in variable inlet pressure mode when increasing the flow rate.

If wikiHow katışıksız helped you, please consider a small contribution to support us in helping more readers like you. We’re committed to providing the world with free how-to resources, and even $1 helps us in our mission. Support wikiHow Yes No Not Helpful 26 Helpful 21

That was a funny project. Some friends and colleagues (Balázs Bobály, Róbert Kormány, Krisztián Horváth) and I were together during a conference and discussing chromatographic questions. One of those was: What happens when coupling two or more columns in series, which have a different number of theoretical plates? read more We were aware of the earlier amazing work of Calvin Giddings on plate heights of non-uniform columns, of Leonid Blumberg’s variance of zone migration in non-uniform medium, and also of Deirdre Cabooter’s works on multi-column systems. However, we were interested in the gradient elution mode (since in practice most separations are performed in gradient mode) and in peak widths: whether the peak width changes if one of the columns that is used for the coupled systems loses a bit of its initial efficiency? If yes, then does the order of the columns impact the apparent efficiency of the system or not? These questions seem to be simple and easy at first sight but the more you think on them the more difficult they become. From here the project went on and katışıksız almost developed by itself kakım questions have arisen. We asked for the help of other prominent colleagues (Davy Guillarme, Santiago Codesido, Gert Desmet) and in the end, some very interesting conclusions were drawn.

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On the other hand, this extreme effect could cause serious issues when transferring previously developed conventional methods to UHPLC method.

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